affinity n. 1.姻親關系 〔cf. consanguinity〕; 密切關系。 2.(語言等的)類似,近似。 3.(男女之間的)吸引力,吸引人的異性。 4.【數(shù)學】仿射性。 5.【化學】親和力。 6.【生物學】類緣,親緣,類同。 have an affinity for children 喜歡小孩。 the close affinity of German with English 德語與英語的密切近似。 affinity card 親和卡〔供小團體內(nèi)部使用的一種信用卡〕。
The binding affinity of remaining hemoglobin sites for oxygen is changed by the presence of co binding . 氧在血紅蛋白其余位置上的鍵合力被一氧化碳所改變。
The high binding affinity and selectivityin conjunction with the polymers ' physical robustness positions molecular imprinted polymers ( mips ) as candidates for use as preliminary screens in drug discovery 聚合物的高親和力、高選擇性及物理性質穩(wěn)定特點使得分子印跡聚合物可用于藥物開發(fā)中的初篩。
Correspondingly , the egf binding affinity of surface egfr and the tyrosine auto - phosphorylation of immuno - precipitated egfr were also enhanced in gntv - s / h7721 cells and reduced in gntv - as / h7721 cells Egfr的信號通路主要有兩條,一是ras - raf - mek一mapk經(jīng)典途徑,另一條是新發(fā)現(xiàn)的pi一3k一pkb途徑。
Detection of antigen - binding affinity of soluble mg7 scfv elisa was adopted to examine the antigen - binding affinity of soluble mg7 scfv ; competitive elisa was performed to test the inhibitory ratio of soluble mg7 scfv to the binding affinity of mg7 mab with its relevant antigen El舊a拐成惴現(xiàn)2個克隆的可容牲mg7seem明顯的抗原結合舌隊其a分別為0 832和0 912 ,均高出附性對照( 0
Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen , the positive dones possessing apparent inhibitory effect were singled out for later use X陽性克到駛知烘扳原kbbjg )濁對陽性克隆進行限制隴臥賜析( uwi和hedlll )鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率,從中j ) ed出對mg7單抗與期??乖Y合有抑余j效應的克隆用于進一涉研究。
The experimental method includes selecting pure complexes of histidine - containing or cysteine - containing materials , from c - and n - terminal group of these amino acids to link to a group which have color or fluorescence or ultraviolet absorption , elucidating their binding affinity , fluorescence or uv - visible spectrum properties with zinc at physiological concentration and to elucidate their structure in the solid state via infrared spectroscopy . with the help of the concerned the data , the analysis was done to prove whether it can be applied to the zinc detection , in other words , whether it can be used as a new fluorescence probe for zinc detection 本實驗首次選用在生物體內(nèi)與zn ~ ( 2 + )鍵合能力很突出的物質? ?組氨酸和半胱氨酸,采用類似于多肽合成的方法,在其羧基或氨基分別嫁接上一個帶有標記的基團,生成穩(wěn)定的共價鍵化合物;在此化合物中模擬生理濃度條件加入鋅離子,通過紅外圖譜、紫外圖譜或熒光圖譜的變化分析鋅離子對標記基團是否產(chǎn)生影響,再結合有關數(shù)據(jù)分析其是否適合檢測鋅離子,即是否可能作為新的鋅離子熒光探針。
Reports about great enthusiasm in the synthesis of the complexes of rare earth medicine , the interaction of the rare earth complexes with dna are very few in the world . it is expected to explore the mechanisms of interaction that are used to offer an opportunity to understand which parameters affect the binding mode and binding affinity to dna . part ii 從這些總結中了解到,人們之所以選擇蘆丁作為小分子配體,是由于蘆丁所具有的特性:它屬于黃酮類化合物,具有獨特的藥用價值及其臨床應用價值,更令人興奮的是它還具有良好的電活性,這樣就可以利用電化學方法來研究其作用機理。
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii ) Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養(yǎng)的mg _ 7雜交瘤細胞中提取并分離mrna ,反轉錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區(qū)基因,并通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產(chǎn)物轉化感受態(tài)tg1大腸桿菌,制備細菌形式的mg _ 7重組噬菌體抗體庫;通過菌落計數(shù)和限制性酶切分析( ecor和hind )評估m(xù)g _ 7重組噬菌體抗體庫的容量和重組率。